Separation of alkaloids by gas chromatography


In the past, the separation of alkaloids from crude alkaloid mixtures has depended upon fractional crystallization, precipitation, countercurrent extraction and either adsorption or liquid phase partition chromatography. Several recent communications have reported the use of gas phase chromatographic techniques for the separation and identification of steroids and high molecular weight fatty primary amines. This communication demonstrates the feasibility of this method for the isolation, separation and identification of alkaloids. Our attention has been focused on alkaloids with molecular weights above 250, since suitable modifications of the conditions should permit separations of lower molecular weight substances without difficulty.



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