Capillary Gas Chromatographic Analysis of Indole Alkaloids: Investigation of the Indole Alkaloids Present in Tabernaemontana divaricata Cell Suspension Culture

Abstract

A capillary gas chromatographic analysis is described by which non-derivatized indole alkaloids and indole-related compounds can be separated and identified when the system is coupled to a mass spectrometer. By the use of this analysis some phenolics and sterols could also be separated. The phenolics coniferyl alcohol and sinapyl alcohol and the sterols campesterol and stigmasterol were identified in Tabernaemontana divaricata cell suspension cultures.